Present study was aimed to evaluate the anti cancer activity of Annona squamosa leaves in selected cell lines in-vitro. Leaves of Annona squamosa were collected from local cultivating areas near to Anantapuramu, shade dried, mechanically made into powder. The powder was subjected to extraction by cold maceration method using water and alcohol mixture as solvent at a ratio of 3:2. The hydro alcoholic extract of Annona squamosa leaves was subjected to preliminary phytochemical screening using standard methods. In-vitro anticancer activity of hydro alcoholic extract of Annona squamosa leaves was performed on LNCap-FGC – Human carcinoma prostate, A549 – Human lung carcinoma and HEK-293 – Human kidney cell lines by MTT assay method at a concentration of 50, 100 and 200 microgram per ml using MTT assay. The percentage yield of Annona squamosa leaves using water: ethanol as solvent (3:2) was found to be 6.45% W/W. Preliminary phytochemical screening of hydro alcoholic extract of Annona squamosa leaves revealed the presence of Proteins, tannins, alkaloids and flavonoids. In anti cancer activity, the plant extract showed IC50 value of 200 mg/ml for all the three tested cell lines that is LNCap-FGC – Human carcinoma prostate, A549 – Human lung carcinoma and HEK-293 – Human kidney.
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